Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: ( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Western Blot, Expressing, Staining

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: YAP1 protein levels are significantly downregulated in the nuclear fractions but not the cytosolic fractions of the AD brains relative to NC brains. Brain lysates were immunoblotted, and protein levels were quantified by normalizing to HDAC2 levels for nuclear fractions and to GAPDH levels for cytosolic fractions. Data are mean ± SEM ( n = 5 per group). *** p < 0.001 by t -test.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques:

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: Antibody-based multiplex microarray screening of 8000 proteins revealed alterations of specific proteins. ( A ) SH-SY5Y cells expressing YAP1-GFP. Scale, 50 µm. ( B ) YAP1-GFP expression supported by immunoblot. ( C ) Examples of glass slide luminescence. ( D ) Heatmap of control and YAP1 biomarkers in 8000 samples. The data were imported to JMP Genomics 10.2 for Hierarchical Cluster analysis.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Multiplex Assay, Microarray, Expressing, Western Blot, Control

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: YAP1 expression increased markers of synaptic endocytosis and ATP-dependent activity. ( A ) Gene ontology (GO) biological process over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) GO cellular component over-representation analysis with 283 differentially expressed biomarkers between control and YAP1-expressing SH-SY5Y cells.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Expressing, Activity Assay, Control

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: ( A ) GO molecular function over-representation analysis with 283 differentially expressed biomarkers between YAP1 and control samples. ( B ) Interaction network of human YAP1 protein as analyzed by STRING.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Control

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: The bar graph shows GO analysis results of the increased ( A ) and decreased proteins ( B ) due to YAP1 expression using ShinyGO 0.85.1. Sets of nonredundant, significant GO enrichment terms are displayed to show the foldenrichment and the number of proteins matched to each term. The fold-enrichment values are displayed with the highest on top, and the shade of each bar reflects the number of proteins found in a given GO category.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Expressing

Journal: Neurology International

Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

doi: 10.3390/neurolint18050096

Figure Lengend Snippet: Evidence of increased levels of ( A ) NEDD9 and ( B ) ARHGEF1 inYAP1-GFP-expressing SH-SY5Y cells when compared to control cells by immunoblotting. ( C ) The LC3-16/LC3-18 ratio was also increased, indicating YAP1 activates autophagy. However, levels of ( D ) p16INK4a, a marker of senescence, were almost completely abolished. Data are mean + SEM, n = 3/group. *, p < 0.05, **, p < 0.01, ***, p < 0.001 by t -test.

Article Snippet: We cultured the immortalized human neuroblastoma cell line SH-SY5Y (cat # CRL2266, ATCC) in DMEM/F12 medium and infected only once with Lenti ORF particles, human YAP1 (mGFP-tagged) transcript variant 1 (cat # RC225864L4V, Origene, Rockville, MD, USA), and generated stable cells expressing YAP1-GFP with puromycin selection at 5.0 μg per milliliter for two weeks.

Techniques: Expressing, Control, Western Blot, Marker

Journal: iScience

Article Title: Promotion of breast cancer by the DNPH1 enzyme

doi: 10.1016/j.isci.2026.115227

Figure Lengend Snippet: Alterations in mammary tumors upon Dnph1 knockout (A) Examples of CD31 staining in mammary tumors from a MMTV-Her2/Dnph1 +/+ and a MMTV-Her2/Dnph1 −/− mouse. Right panels show ImageJ-mediated image modulation to enhance vessel structures, allowing the computer-mediated quantification of blood vessel density. Scale bars, 0.4 mm. (B) Corresponding quantification of vessel density. Tumors were age- and weight-matched; two-tailed, unpaired t test. (C) Tumor vessel density plotted against the weight of the tumor. These tumors were not weight- or age-matched, yet include those shown in panel B. Shown are linear regressions; while the slopes were not significantly different, the Y-intercepts were significantly different ( p = 0.0007). (D) Relative levels of AMP, GMP, and UMP in mammary tumors from MMTV-Her2/Dnph1 +/+ and MMTV-Her2/Dnph1 −/− mice; two-tailed, unpaired t test. (E and F) Immunohistochemical staining for phospho-AMPKα or phospho-YAP1, respectively, on age- and weight-matched tumors as in panel B. Examples of immunohistochemical staining are shown at the bottom. Scale bars, 0.4 mm. Means with standard deviation are shown in panels B and D-F.

Article Snippet: YAP1 phosphorylated on serine 127 , Cell Signaling , Cat# 4911.

Techniques: Knock-Out, Staining, Two Tailed Test, Immunohistochemical staining, Standard Deviation

Journal: iScience

Article Title: Promotion of breast cancer by the DNPH1 enzyme

doi: 10.1016/j.isci.2026.115227

Figure Lengend Snippet: Model how DNPH1 promotes breast tumor development and progression This model is centered around a DNPH1-YAP1 axis, yet DNPH1 may utilize to-be-identified effectors other than YAP1 to stimulate, e.g., stem cells or angiogenesis.

Article Snippet: YAP1 phosphorylated on serine 127 , Cell Signaling , Cat# 4911.

Techniques: